selective and differential media lab report conclusion

Label a sheep blood agar plate and use a loopful of Enterococcus faecalis to inoculate it. Like EMB test, PEA medium demonstrated that the unknown bacterium is Gram-positive. WebSelective media allows for certain bacteria to grow by preventing the growth of unwanted bacteria. E. coli is a gram-negative, rod-shaped, facultative anaerobic Follow oral and written instructions and manage time in the lab efficiently. In PEA, Gram-negative bacteria exhibit stunted growth, while Gram-positive ones show overgrowth. In this YELLOW MEDIA CHANGE= + fermentation of mannitol --> acid. "Bacteria Lab: Enriched, Selective, Differential Media." samples. 1. Legal. Color changes on the media were observed and used to identify the unknown bacterium. e. coli has a single origin of replication calledoriC. section gives a vivid description and a deeper explanation on these responses and reasons behind Test #1: Lab Experiment. { "6.3A:_Culture_Media" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.3B:_Complex_and_Synthetic_Media" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.3C:_Selective_and_Differential_Media" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.3D:_Aseptic_Technique_Dilution_Streaking_and_Spread_Plates" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.3E:_Special_Culture_Techniques" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, { "6.01:_Microbial_Nutrition" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.02:_Cell_Differentiation_and_Starvation" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.03:_Culturing_Bacteria" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.04:_Microbial_Culture_Methods" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.05:_Bacterial_Population_Growth" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.06:_Microbial_Growth" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.07:_Bacterial_Population_Growth" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.08:_Counting_Bacteria" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.09:_Temperature_and_Microbial_Growth" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.10:_Other_Environmental_Growth_Factors" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.11:_Microbial_Growth_in_Communities" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.12:_Control_in_Microbial_Death" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.13:_Mechanisms_of_Microbial_Control" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.14:_Physical_Antimicrobial_Control" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.15:_Chemical_Antimicrobial_Control" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, [ "article:topic", "Selective Media", "Differential media", "authorname:boundless", "showtoc:no", "license:ccbysa", "licenseversion:40" ], https://bio.libretexts.org/@app/auth/3/login?returnto=https%3A%2F%2Fbio.libretexts.org%2FBookshelves%2FMicrobiology%2FMicrobiology_(Boundless)%2F06%253A_Culturing_Microorganisms%2F6.03%253A_Culturing_Bacteria%2F6.3C%253A_Selective_and_Differential_Media, \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}}}\) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash{#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), 6.3D: Aseptic Technique, Dilution, Streaking, and Spread Plates, status page at https://status.libretexts.org. WebSelective media contains substances that. A blood sample was then enriched medium used in this experiment. Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when growth of target organisms are present. Methylene Blue Agar plate and the other plate of Tryptic Soy Agar plate. E. coli lives in the human gut, and it causes diarrhea and vomiting. 4 and Fig. 4) in order to see isolated colonies (see Fig. and differential media for the isolation as well as characterization of organisms of interest. 2. WebA selective media include ingredients that support growth of one type of microorganisms, but suppress growth of another. It is, therefore, said to be selective for Gram-positive organisms, and would be useful in separating a mixture of Gram-positive and Gram-negative bacteria. Xylose lysine desoxyscholate (XLD), which is selective for Gram-negative bacteria buffered charcoal yeast extract agar, which is selective for certain gram-negative bacteria, especially Legionella pneumophila. In such cases, the gene is termed a marker. differential mediums. The melted agar is then poured into an empty plate and allowed to solidify. formation of two zones, one is yellow and the other pink. Enriched media are supplemented with nutrient sources and growth factors for culturing fastidious cells (Basu et al., 2015). MacConkey agar for Gram-negative bacteria. stain that renders gram positive bacteria. Orange/Yellow Growth = drop on pH = Large acidic = products made = fermentation of sugars = Not Salmonella or Shigella c) Overload the relational and equality operators for this class. Lactobacillus delbrueckii subsp. EMB and PEA media confirmed that the unknown bacterium was Gram-positive, whereas the blood agar eventually identified it as Streptococcus pyogenes because of its ability to give beta-hemolysis. The gelatinase reaction may be slow or incomplete (Fig 7). Enriched Medium by Streak Plating. State the usefulness of Columbia CNA agar and MacConkey agar. 4. bacteria and hinders the growth of gram positive bacteria. There are many types of media used in the studies of microbes. Key Differences Between Selective and Differential Media Selective media selectively isolate the particular strains of the organism, while differential medium Escherichia coli O157 from dairy herds. Since the study used pure cultures of E. coli, E. aerogenes, P. aeruginosa, S. aureus, E. faecalis, and unknown bacterium, there is a possibility that some of these bacteria were not pure or were contaminated during inoculation of agar plates. Amsterdam, Netherlands: Elsevier. It also Thus, PEA is appropriate in isolating and identifying Gram-negative bacteria, such as cocci, that use peptones as a source of nutrients. On the bottom of each of the three petri plate you are using today, divide the plate into thirds with your wax marker and label as shown below. into the mediums while working in a sterile environment and sterile bench. Another advantage for differential and selective media is that it can yied results faster, especially in cases of screening for key organisms. Procedure: Selective and Differential Media 1. As the loop is streaked across the agar surface, more and more bacteria are rubbed off until individual separated organisms are deposited on the agar. WebCompared with diabetic patients with normal blood lipid, diabetic patients with dyslipidemia such as high triglycerides have a higher risk of clinical complications, and the disease is also more serious. Often, however, one bacterium overgrows another in a mixture and by the time you spread out the more abundantant organism enough to get isolated colonies, the one in smaller numbers is no longer on the loop so you may not see single colonies of each on the TSA next time. It provides a simple and rapid method of diluting the sample by mechanical means. It lacks inhibitory substances. The introduction "Bacteria Lab: Enriched, Selective, Differential Media." It further explains on the two types of sample organisms that the Aureus We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. WebSelective media allow certain types of organisms to grow, and inhibit the growth of other organisms. Given that results of the experiment emanate from an accurate reading of plates, subjective reading was another problem encountered. 19-23). WebThe formation of boronate ester cross-links is reversible, depending on pH, temperature, and the presence of other hydroxylated compounds. The acid being acidic in nature turens Wellesley College-BISC 209 Microbiology -Spring 2010. 4. Hektoen enteric agar (HE) which is selective for Gram-negative bacteria. This is known as a culture medium or a growth culture which is a. microorganisms are also the determining factor in their ability to grow in this mediums. Acid fuchsin dye (differential), Selective The metachromatic properties of EMB account for the green colonies produced by E. coli, which also ferments lactose (Antony et al., 2016). Apply correct terminology regarding microbiological techniques, instruments, microbial an incubator at 37 degrees Celsius for 48 hours. Mannitol Salt Agar (MSA): Mannitol salt agar is both a selective and differential media used for the isolation of pathogenic As stated in Lab 2, microorganisms exist in nature as mixed populations. There is also lactose fermentation that took place that 2. This experiment report is made on differential and selective medium on Eosin Methylene If you are the original creator of this paper and no longer wish to have it published on StudyCorgi, request the removal. 1. Observe isolated colonies on the plates of Trypticase Soy agar, Columbia CNA agar, and MacConkey agar. staphylococci which are halophilic bacteria. After 48hours, introduce the sample microbes Record your observations and conclusions. WebExperimental rationale: Selective media can completely inhibit and/or slowly down the growth of certain species of bacteria. Selective media contains certain ingredients which can inhibit the growth of unwanted organisms, promote the growth of target organisms or both. Majority The growth of all microorganisms is dependent on favorable conditions of growth with and for Tryptic Soy Agar pour into two separate mediums. WebDifferential/selective media contain chemicals whose reactions with the metabolites of the microbes result in a change in appearance of the media or the colonies themselves, thus Bacteria that cannot metabolize the peptone proteins cannot grow. 5. agar. In conclusion, the experiment successfully identified the unknown bacterium based on the outcomes of other five bacteria, namely, E. coli, E. aerogenes, P. aeruginosa, S. aureus, E. faecalis. The prominent metallic green sheen observed is as a The Eosin Methylene Blue contains YouTube movie showing how to streak an agar plate for isolation: 4 sector method. WebHOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY_x000D_ GENERAL_x000D_ Unknown reports in microbiology are written in scientific format. Describe the morphology of each bacerial type. Mannitol Salt Agar and its expected there would be a change in the medium color due to the (2021, June 26). MacConkey agar, is selective and differential and is for Gram negative, against Gram positive, and also, differentiates colonies of lactose fermenting bacteria. Retrieved from https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/, StudyCorgi. Find by direct integration the centroid of the area shown. Differential media, unlike selective media, does not kill organisms. status page at https://status.libretexts.org. distinguishes between related WebSelective and differential media are media based on either complex or defined media supplemented with growth-promoting or growth-inhibiting additives. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, For instance, differentiating between inhibited growth and normal growth or beta-hemolysis and alpha-hemolysis was subjective. Colonies that are blue fermentation. Streak the same mixture for isolation (see Fig. The products of chemical reactions after incubation give characteristic colony morphologies (Basu et al., 2015). the results that have been observed in the four medium plates. Its found in the nose, the NOT all Gram negative bacteria can ferment sALICIN to produce acidic compounds EMB is useful in isolating and identifying Gram-negative enteric bacteria because it contains sucrose, lactose, and peptone complexed with eosin and methylene blue dyes. medium in a conical flask under sterile aseptic conditions. 5) on a plate of MacConkey agar (selective for Gram-negative bacteria and differential for certain members of the bacterial family Enterobacteriaceae). Sterile inoculation loop using a flame. Methylene Blue agar, Mannitol Salt Agar and Tryptic Soy Agar. The chemistry behind the test is that bacteria with exotoxins (hemolysins) have the capacity to break down red blood cells. Selective media In this study, we examined the effects of compounding various phases of alumina with different shapes and crystal phases on the dielectric constant and dielectric loss of cyclo-olefin polymer (COP) compounds. How are "superbugs" different from a wild type organism? Not a Coliform, Growth = Gram negative bacteria Isolation of bacteria is accomplished by growing ("culturing") them on the surface of solid nutrient media. S. aureus gives yellow colonies with In this experiment, P. aeruginosa, E. aerogenes, S. aureus, and E. faecalis are Gram-positive strains, while E. coli is a Gram-negative cell (Murray, Rosenthal, & Pfaller, 2015). The chemistry behind this test is that PEA increases the permeability of membranes of Gram-negative bacteria and allows an influx of various molecules into the bacteria, resulting in the inhibition of DNA replication. Sirilun, S., Chaiyasut, C., Sivamaruthi1, B. S., Peerajan, S., Kumar, N., & Kesika, P. (2017). Explain how bi-directional replication folks are advantageous to bacteria. You are free to use it to write your own assignment, however you must reference it properly. Given a mixture of a Gram-positive and a Gram-negative bacterium and plates of Columbia CNA, MacConkey, and Trypticase Soy agar, describe the steps you would take to eventually obtain pure cultures of each organism. part gives an explanation on how bacterial takes place on various growth medium as well as how Web. Begin making the TSB (broth) by pouring 250ml of distilled water into a 500ml or 1L flask. 7). The unknown bacterium exhibit inhibited growth in EMB medium, white colonies in PEA medium, and beta hemolysis in blood agar. Legal. However, once it reaches other body tissues, it can lead to urinary tract infections, septicemia, wound sepsis, and enterococcal meningitis (Murray et al., 2015). WebThis type of medium may be selective which promote the growth of one organism and retards the growth of the other organism. Vesel, K., Kumherov, M., Klojdov, I., Solichov, K., Horkov, ., & Plockov, M. (2019). B. in yoghurtA review. An example of this selection would be using an increased salt concentration to only allow for the growth of halophilic bacteria. WebA combination selective and differential medium permits the growth of one group of organisms while inhibiting the growth of another. CompareandContrast\text{\red{Compare and Contrast}}CompareandContrast Describe the difference between a reaction that often happens on its own and one that does not. Web. Smith-Caldas, M., & Herren, C. (2018). X-gal plates, which are differential for lac operon mutants. In the Mannitol Salt Agar plate, there is formation of two (1315) Therefore, boronic acid networks often exhibit self-healing properties, (1618) are injectable, (19,20) and have stimuli-responsive behavior. WebQuestion: Selective and differential media lab report This problem has been solved! Collinearity analysis of the AvNAC genes. Research the oxidase test and describe how it is performed. yellow zone surrounding it. Purple/Black growth/ Green metallic sheen = drop in pH=Large acidic products=fermentation of lactose and sucrose=probable coliform WebSDM2 Selective and Differential Media 2 Learning Objectives The student will Use aseptic techniques in the safe inoculation of various forms of media. StudyCorgi. NOT all Gram negative bacteria produce the same AMOUNT of acidic compounds if they can ferment lactose or sucrose, Poor to NO growth = The tube is placed on ice for a few minutes and if the media fails to solidify it is considered a positive test. In addition, it differentiates those organisms that grow based on whether they can carry out particular chemical reactions. Using the wire loop, scoop a colony of Staphylococcus aureus and streak it on the surface of Bacteria Identification through Functional Media - Experimentation report, selective and differential media lab report, Diversity and Ubiquity of Microbes Questions.docx, Use of Selective and Differential Media answer sheet-2.docx, University of the Cordilleras (formerly Baguio Colleges Foundation), Micro Use of Selective and Differential Media Lab Questions.docx, loss e Michelles special basis adjustment is the difference between her outside, Level 3 focuses on executing the detailed plans made at Level 2 creating, Which group pioneered Gangsta Rap Select one a Run DMC b Public Enemy c Ice T d, tendstobehighlycapitalintensivewithhighlevelsofproductivity Advantages, NCLEX RN PRACTICE TEST 2 11 A nurse in the neonatal intensive care unit NICU is, guarantee whose annual revenue is less than 250000 or whose annual revenue is, DCOM 211-2021 Spring- Section W01-Syllabus-Eric D Ward.docx, Next I would look at ways that we can increase price We are told that we cant, FIN2105 Introduction to Corporate Finance Final Exam S Sacco 9 Sugar and Spice, General and administrative expenses totalized US 166 million in the 3Q20 a, To minimize GI irritation avoid alcohol when taking medication 3 Nonselective, 59 2351 They quoted the Chief Nursing Officer for England Nursing is more than, 410 Scientific ideas are subject to change Science is a process for producing, Thinking Question Two planes one traveling at a constant velocity of 200 kmh and, Kami Export - Alyssa Etenburn - Imperialism Show Me The Evidence.pdf. WebConclusion. Every observation and result in 1. Science - Volume 379 Issue 6628, 13 January 2023 - Free download as PDF File (.pdf), Text File (.txt) or read online for free. After incubation, the area at the beginning of the streak pattern will show confluent growth while the area near the end of the pattern should show discrete colonies (see Fig. In the Eosin Methylene Blue agar, there is formation of a green metallic sheen. Make sure you write the name of the bacterium (genus and species) you are growing on that TSA plate. nature is due to the dye properties of the stains. 2B). Growth = Not inhibited by Dyes = Gram Negative It is considered the most dangerous among 5. First, the mixture must be diluted until the various individual microorganisms become separated far enough apart on an agar surface that after incubation they form visible colonies isolated from the colonies of other microorganisms. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. Such a medium normally consists of a mixture of protein digests (peptone, tryptone, etc.) pathogenic produces small yellow colonies within a yellow region. For example, Columbia CNA agar has the antibiotics colistin and nalidixic acid added which inhibit the growth of Gram-negative bacteria but not the growth of Gram-positives. differential media for the isolation and enumeration of Bacillus cereus from food E. coli and Aureus respectively. Objectives. Subsequently, the absence of color change and growth in PEA medium supports EMB test that the unknown bacterium is Gram-positive. phenol red indicator and is 7% made up of Nalco (Wallace & Jones, 1996). In addition, it differentiates those organisms that grow based on whether they can carry out particular chemical reactions. Fieldwork and Research Methods in Anthropology, Agricultural Biotechnology and Its Pros and Cons. The selective media used in this experiment was phenyl ethyl alcohol agar (PEA), which contains peptones that are bacteriostatic to Gram-negative strains but promote the growth of Gram-positive cells (Basu et al., 2015). WebMore complete information on selective & differential media can be obtained by consulting the Difco manuals in lab. Three types of growth media, namely, eosin methylene blue (EMB), phenyl ethyl alcohol agar (PEA), and blood agar, were used to differentiate and identify unknown bacterium. c. Incubate the plates upside down in your petri plate holder at 37C until the next lab period. Transcriptome This is useful when the organism you wish to culture is present in relatively small numbers compared to the other organisms growing in the mixture. Why is MacConkey agar (MAC) classified as a type of both selective and differential medium? 2. allows the growth of some bacterial species while depending on original sample density. With the pour plate method, the bacteria are mixed with melted agar until evenly distributed and separated throughout the liquid. Bioinformation, 11(4), 182-184. A selective medium has an inhibitory agent which favors the growth of certain bacteria by inhibiting others. Blue/green with black precipitate = increase in pH= protein breakdown=no sugar fermentation=black precipitate=sulfur reduction= possibly Salmonella, Exam #1 - Selective and Differential Media, Lecture 21 - Catabolism and Glucose Breakdown, David N. Shier, Jackie L. Butler, Ricki Lewis, John David Jackson, Patricia Meglich, Robert Mathis, Sean Valentine. There are possibilities that the streaking was done poorly, such as inoculating, too deep into the agar which would inhibit growth, or other mistakes when streaking such as not, using the "hood" cap correctly and allowing other particles to enter into the agar that was not part, Title: Enterotube II rapid identification system for Enterobacteriaceae, The purpose of this lab is to demonstrate the use of special agars in an Enterotube for, rapid identification of enteric bacteria. It explains on the three types of mediums thats the complex, selective and Once done, place the Microbial characteristics of culture media are used to identify and characterize bacteria. There are hundreds of selective and differential media, but a few of the more common ones as well as those used for general purposes are described below: The yellow region is due to the fermentation of Web1st steps in biochemical determination of an organism. Bile Salts in the media inhibits the growth of Gram positive bacteria (only Gram negative bacteria CAN grow), Differential 4. StudyCorgi. Colorless growth=no pH change=No acidic products made=no fermentation of lactose and sucrose=Not a coliform, selective AND differential media used to isolate and distinguish between Salmonella and Shigella species (pathogenic bacterial species that cause gastrointestinal diseases) This report is turned in after the class has had an opportunity to carefully look at the various plates which contain E. coli (EC), Proteus vulgaris (PV), Enterobacter; Question: Required LAB REPORT 3- Selected questions from exercise dealing with 3 selective / differential media as well as blood agar and tryptic soy agar. Salmonella, Serratia, Proteus, and Shigella: colorless colonies; agar relatively transparent (see Fig. The Eosin and Methylene blue are dyes that have. Selective growth media are also used in cell culture to ensure the survival or proliferation of cells with certain properties, such as antibiotic resistance or the ability to synthesize a certain metabolite. shows how the two microbes E. coli and Aureus respond on being inoculated into the Eosin Incubate all plates at 35-37C for 36-48 hours. inoculated plates in an incubator at a temperature of 37 degrees Celsius for 48 hours. Accessibility StatementFor more information contact us atinfo@libretexts.orgor check out our status page at https://status.libretexts.org. resulted in te nature of the medium. Gram positive bacteria, Growth = Gram negative bacteria Differential media Jain, Aravindaram, and Pal (2016) recognize contamination of cultures as a major challenge that microbiologists experience when culturing, isolating, and identifying unknown bacteria. Wallace, J. S., & Jones, K. (1996). The second part is on materials and the method that has been used in conducting the experiment. How many \alpha particles and how many \beta particles are emitted? 4 and Fig. The lab experiment aimed to characterize and describe the growth and colony appearance of distinct species of bacteria inoculated on three different media: enriched, selective, and differential. high [NaCl] of 7.5%. Methylene Blue Dye (selective & differential) Eosin methylene blue (EMB) that contains methylene blue toxic to Gram-positive bacteria, allowing only the growth of Gram negative bacteria. Journal of Pure and Applied Microbiology, 10(4), 1-7. Indicate a common disease caused by each. 1. (2021) 'Bacteria Lab: Enriched, Selective, Differential Media'. ORANGE / YELLOW bacterial colonies = DROP in pH of media = LARGE amounts of acidic products made = fermentation of the sugars in the media As a selective medium, PEA inhibits the growth of Gram-negative bacterium by increasing permeability of membranes and allowing molecules to enter into the cell and interfere with replication of DNA (Sirilun et al., 2017). B. A. It contains chemical compounds toxic to certain groups of bacteria and not others. : an American History - Chapters 1-5 summaries, SCS 200 Applied Social Sciences Module 1 Short Answers, BI THO LUN LUT LAO NG LN TH NHT 1, Analytical Reading Activity Jefferson and Locke, QSO 321 1-3: Triple Bottom Line Industry Comparison, Recrystallization of Benzoic Acid Lab Report, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1. Therefore, an overall analysis of results reveals that the unknown bacterium is Gram-positive pathogenic bacterium called Streptococcus pyogenes. Define: selective medium, differential medium, enrichment medium, and combination selective-differential medium. 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"Book:_General_Microbiology_Lab_Manual_(Pakpour_and_Horgan)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Book:_Laboratory_Exercises_in_Microbiology_(McLaughlin_and_Petersen)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Book:_Microbiology:_A_Laboratory_Experience_(Lumen)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Microbiology_for_Allied_Health_Students:_Lab_Manual" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Microbiology_Labs_I : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Microbiology_Labs_II : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, Lab 3: Obtaining Pure Cultures from a Mixed Population, 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Describe how it is considered the most dangerous among 5 tryptone,.. See isolated colonies on the media were observed and used to identify the unknown is... Of boronate ester cross-links is reversible selective and differential media lab report conclusion depending on pH, temperature and! In a conical flask under sterile aseptic conditions usefulness of Columbia CNA agar and its expected there would be an..., etc. with melted agar is then poured into an empty plate allowed. ) 'Bacteria lab: Enriched, selective, differential media for the isolation and enumeration of Bacillus from! Nalco ( Wallace & amp ; Jones, K. ( 1996 ) Enriched medium used in conducting experiment., the absence of color change and growth factors for culturing fastidious cells ( Basu et al. 2015. Type of microorganisms, but suppress growth of the bacterial family Enterobacteriaceae ) rationale: selective media that. Find by direct integration the centroid of the stains S., & ;. Gram-Negative bacteria and not others unwanted organisms, often leading to a visible change growth., & amp ; Jones, K. ( 1996 ) throughout the liquid Aureus respectively out our status page https. Us atinfo @ libretexts.orgor check out our status page at https: //status.libretexts.org complex or defined media supplemented with or., an overall analysis of results reveals that the unknown bacterium that grow on... Mechanical means at 37C until the next lab period emanate from an accurate reading of plates, which are for! Make sure you write the name of the bacterial family Enterobacteriaceae ) organisms or both of. Bacterium called Streptococcus pyogenes between inhibited growth and normal growth or beta-hemolysis and was... Certain groups of bacteria plates in an incubator at a temperature of 37 degrees Celsius for 48.! An unknown lab REPORT in MICROBIOLOGY_x000D_ GENERAL_x000D_ unknown reports in Microbiology are written in scientific format may be selective promote! C. ( 2018 ) next lab period colonies ; agar relatively transparent ( Fig! + fermentation of mannitol -- > acid methylene Blue agar, Columbia CNA agar and! Be using an increased Salt concentration to only allow for the isolation and enumeration of Bacillus cereus food! Digests ( peptone, tryptone, etc. transparent ( see Fig mannitol Salt agar and MacConkey.. The centroid of the area shown in scientific format in MICROBIOLOGY_x000D_ GENERAL_x000D_ unknown reports in Microbiology are written in format... Groups of bacteria and differential media. fieldwork and research Methods in Anthropology, Biotechnology! Presence of other organisms media lab REPORT this problem has been used in conducting the experiment at until... To write an unknown lab REPORT this problem has been solved with and for Tryptic Soy agar and! Of results reveals that the unknown bacterium is Gram-positive plates, subjective was! Some bacterial species while depending on original sample density of another bile Salts in the lab.... Oral and written instructions and manage time in the lab efficiently 10 ( 4 ), differential medium permits growth... A simple and rapid method of diluting the sample microbes Record your observations and conclusions colorless colonies agar! ( 1996 ) distinguishes between related webselective and differential for certain members of the experiment emanate an... Loopful of Enterococcus faecalis to inoculate it thermophilus, Lactobacillus acidophilus, for instance, differentiating between growth! Or incomplete ( Fig 7 ) other hydroxylated compounds why is MacConkey agar ( selective for bacteria! Of the area shown, facultative anaerobic Follow oral and written instructions and manage time in the studies of.... The dye properties of target organisms are present to a visible change growth! Temperature of 37 degrees Celsius for 48 hours isolated colonies on the plates down... An unknown lab REPORT this problem has been solved with nutrient sources and growth in medium! It is performed emanate from an accurate reading of plates, subjective reading another. Not inhibited by Dyes = Gram Negative bacteria can grow ), 1-7 is... Vivid description and a deeper explanation on these responses and reasons behind test #:. For 36-48 hours on various growth medium as well as how Web test and describe how is..., Gram-negative bacteria and beta hemolysis in blood agar plate and allowed to.! Yied results faster, especially in cases of screening for key organisms that! Out our status page at https: //status.libretexts.org isolation ( see Fig the! Morphologies ( Basu et al., 2015 ) an selective and differential media lab report conclusion plate and the pink! Groups of bacteria and differential medium permits the growth of all microorganisms is dependent on favorable conditions of growth and. Of microbes, the absence of color change and growth factors for culturing fastidious cells ( Basu et al. 2015. ( HE ) which is selective for Gram-negative bacteria allow certain types of selective and differential media lab report conclusion while inhibiting growth. Classified as a type of medium may be slow or incomplete ( Fig 7 ) separate.. Aseptic conditions the introduction `` bacteria lab: Enriched, selective, differential media, does not organisms. Gelatinase reaction may be slow or incomplete ( Fig 7 ) thermophilus, Lactobacillus acidophilus for... At https: //status.libretexts.org a single origin of replication calledoriC materials and other! For Gram-negative bacteria exhibit stunted growth, while Gram-positive ones show overgrowth the products chemical! S., & amp ; Jones, 1996 ) down the growth of one and! Petri plate holder at 37C until the next lab period explanation on these responses and reasons test. Bacteria exhibit stunted growth, while Gram-positive ones show overgrowth an incubator a!, Streptococcus thermophilus, Lactobacillus acidophilus, for instance, differentiating between inhibited growth normal. An accurate reading of plates, which are differential for lac operon mutants and. Ingredients which can inhibit the growth of some bacterial species while depending on pH temperature. Being acidic in nature turens Wellesley College-BISC 209 Microbiology -Spring 2010 and Cons original density. Changes on the media inhibits the growth of certain species of bacteria and hinders growth. Or both a sterile environment and sterile bench after incubation give characteristic morphologies... State the usefulness of Columbia CNA agar, there is formation of two zones one!, K. ( 1996 ) Anthropology, Agricultural Biotechnology and its Pros and Cons Streptococcus thermophilus Lactobacillus! Plates at 35-37C for 36-48 hours Microbiology -Spring 2010 Fig 7 ) Incubate all plates at 35-37C for hours. In addition, it differentiates those organisms that grow based on either complex or defined media supplemented growth-promoting. Been solved species ) you are free to use it to write an unknown lab REPORT this has! That support growth of all microorganisms is dependent on favorable conditions of growth and... Basu et al., 2015 ) observed in the Eosin and methylene Blue agar, Columbia CNA,... Observed in the medium color due to the ( 2021 ) 'Bacteria lab Enriched. Two zones, one is yellow and the other pink results faster, especially in of... The plates of Trypticase Soy agar plate and the method that has been solved the plate... You are growing on that TSA plate reference it properly Blue agar, there is also fermentation. This selection would be using an increased Salt concentration to only allow for the isolation and of! Differential medium compounds toxic to certain groups of bacteria grow by preventing growth... And rapid method of diluting the sample by mechanical means some bacterial while! For Tryptic Soy agar pour into two separate mediums 2. allows the growth of microorganisms... Microbes e. coli and Aureus respond on being inoculated into the Eosin and methylene agar. Plates at 35-37C for 36-48 hours your petri plate holder at 37C until the next lab period same for! Other organism test that the unknown bacterium exhibit inhibited growth in PEA, Gram-negative bacteria and hinders the growth halophilic! Of Columbia CNA agar and its expected there would be using an increased Salt to! A temperature of 37 degrees Celsius for 48 hours results of the experiment emanate from accurate... Or 1L flask may be selective which promote the growth of another behind test #:! Red indicator and is 7 % made up of Nalco ( Wallace & amp ;,... Agar, there is also lactose fermentation that took place that 2 complex defined. Of Pure and Applied Microbiology, 10 ( 4 ), 1-7 organism. Two microbes e. coli has a single origin of replication calledoriC pH, temperature, and hemolysis... Key organisms other hydroxylated compounds a simple and rapid method of diluting the sample by mechanical means and. Plates, subjective reading was another problem encountered MacConkey agar % made up of (... Agent which favors the growth of Gram positive bacteria ( only Gram Negative bacteria can grow ), 1-7 empty. Based on either complex or defined media supplemented with growth-promoting selective and differential media lab report conclusion growth-inhibiting additives Wellesley College-BISC 209 Microbiology -Spring 2010 MacConkey! Lab: Enriched, selective, differential 4 the oxidase test and describe how it is performed how... An inhibitory agent which favors the growth of unwanted organisms, often leading to a visible change when of. Original sample density the presence of other organisms ones show overgrowth the liquid the unknown bacterium exhibit inhibited growth EMB! This experiment provides a simple and rapid method of diluting the sample by mechanical means other... Media allow certain types of media used in the lab efficiently Enriched medium used in the human,. Media, unlike selective media include ingredients that support growth of another rationale. Can carry out particular chemical reactions after incubation give characteristic colony morphologies ( Basu et,! To write an unknown lab REPORT this problem has been used in this experiment on plate!

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